The father who never returned - S2.E4

Mikage decides to investigate, she runs across the swordwoman and just in time to save Ayunosuke from falling. After restoring her health, Jiyu blows the whistle and asks Ayunosuke about the Eyepatch. Ayunosuke explains everything, and though Jiyu is moved with tears, she refuses to accept the Eyepatch, swearing that she will never become Yagyu Jubei again.