Diego and Alicia find out about tree frogs once Diego learns that there are tree frogs in trouble. Alicia figures out that they are Red-Eyed Tree Frog's, and Diego figures out that the wind blew the tree frogs off of their tree and they are going down the river. Alicia tries to find the tree fogs a new home while Diego and Baby Jaguar try to save them. Along their way, they face several new challenges, such as finding a way to get across a muddy pit, jumping over rolling coconuts, finding their way into a pyramid that the tree frogs went into, and finding their way through the pyramid to get to the tree frogs.