Real World/Road Rules Challenge 2000 is the 3rd season of the MTV reality game show, The Challenge. The season is directly subsequent to Real World/Road Rules Challenge the season.

Real World/Road Rules Challenge 2000 is the second six-on-six The Challenge in the series. The teams traveled via tour bus starting in Las Vegas, Nevada then moving to the eastern U.S., ending in Miami, Florida after competing in different individual challenges. Each time a team won an individual challenge, they won $10,000. After each mission won, the winning team would invest those 10K in the product of their choice through E-Trade. At the end, the winning team also won any money won in the stock market. The last mission would be for the right to keep the money collected in the pot, an additional cash prize and a car. This season also featured an additional challenge where the two teams had to give up smoking for the duration of filming. If any cast member on either team were caught smoking, their cast would lose the additional challenge.