Johnny Test is a Canadian/American animated television series. It premiered on Kids' WB, on The WB Television Network, on September 17, 2005. The series currently airs in the United States on Cartoon Network, as of January 7, 2008, and in Canada on Teletoon, as of October 28, 2006. The show was produced by Cookie Jar Entertainment for its second and later seasons. The series is rated TV-Y7 for the first four seasons, and TV-Y7-FV for the fifth.

Johnny Test started its second season on October 28, 2006 with "Hoist the Johnny Roger" and "Johnny's Turbo Toy Force" and ended on May 12, 2007 with episodes "The Good, the Bad, and the Johnny" and "Rock-a-Bye Johnny".

This season, along with its first season, was released on DVD in a bundle on February 15, 2011 in Region 1.

This season marks the first appearance for Ashleigh Ball as the new voice of Mary Test, Sissy Blakely, and Missy.